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EB probed with 2 pre-immunization sera; Sera gen, are shown in Fig. Mice immunized i. T-cells 3. Proliferation was determined The body weight of the mice was measured daily following the from the incorporation of [3 H]thymidine.
As shown in Table 2, in i. Table 2 T-cell proliferative responses of immunized mice from the day before the i.
Percentage change in mean body weight of mice following the i. By the 4th day after the i. This group of mice quickly recovered most of their weight and by day 10 Fig.
Number of C. Dots represent individual animals and the horizontal bars correspond to the medians for the different groups. Recovery of C. The lungs were homogenized and the number of chlamydial 3. In the positive control group immunized i. For the negative con- response. The mean weight of the lungs from the positive con- trols groups inoculated with MEM-0 the median IFU count was trol mice vaccinated with live EBs was 0. However, the animals immunized with nMOMP tive control group 0.
The fact that A does not improve the protection had IFU counts below the limit of detection. For the group vac- afforded by rMOMP argues against a general adjuvant effect.
Therefore, one could possibly argue that the protein pre- sented to the immune system may more closely resemble the native structure when nMOMP is trapped with A than when it is solu- 4. On the to the fate of nMOMP following inoculation. Protein-bound deter- other hand, no difference in protection was observed between the gent, indeed, is in a constant and rapid equilibrium with the rMOMP preparations formulated with either Z or A This solution.
Membrane protein—APol complexes, on the other hand, are system, rather than from an adjuvant effect of A This could make for whole pathogens [39,40]. In addition, vaccines containing T cells remains controversial [51—53]. Miller et al. The authors proposed immunogens, poses structural challenges.
Antibod- the secondary, tertiary and quaternary structures need to be native- ies to conformational epitopes are also thought to be critical for like since they are critical for inducing protection [47,48].
Several groups have now nor N-linked glycosylated [30]. For example, Musson et al. They showed that the degree of process- structure, the secondary and tertiary structures of rMOMP appear ing was dependent on the localization of the epitopes.
Epitopes for the most part disordered. Its quaternary structure, if any, processing. These structural differences pH, and did not require proteolytic processing. Also, Hanada et al. They [59] proposed that the peptide was produced several origins. In our vaccine formulation amphipols are play- by post-translational excision in the cytosol followed by ligation.
Chlamydia trachomatis infections of the adult. Sexually transmitted diseases. The potential for vaccine against infection of the genital transmembrane peptides to preformed membranes, be they lipid tract with Chlamydia trachomatis. Sex Transm Dis ;5 April—June 2 —7. Chlamydial disease pathogenesis.
The kD chlamydial hypersensitivity antigen is a stress response protein. J It is conceivable that, in so doing, they modulate the way the anti- Exp Med ; October 4 — Structural and func- tional analyses of the major outer membrane protein of Chlamydia trachomatis. This may be particularly important for cysteine-rich proteins such J Bacteriol ; September 17 — Diversity class I- and II-restricted presentation and processing of antigens of Chlamydia trachomatis major outer membrane protein genes.
Pre- formulated with APols and nMOMP, an integral membrane pro- diction of the membrane-spanning beta-strands of the major outer membrane protein of Chlamydia. Protein Sci ;11 July 7 — Vaccination with the Chlamydia tra- same antigen formulated with a detergent. This enhancement of chomatis major outer membrane protein can elicit an immune response as the protective ability of nMOMP most likely results from a better protective as that resulting from inoculation with live bacteria.
Infect Immun ;73 December 12 — Several subunit vaccines currently include integral membrane oligodeoxynucleotides as an adjuvant induces a protective immune response against an intranasal chlamydial challenge.
Infect Immun ;70 September proteins [47,48]. The possibility that the protective ability of these 9 —7. Chlamydia trachomatis native major outer membrane protein induces partial protection in nonhuman primates: implication for a trachoma transmission- Acknowledgements blocking vaccine. J Immunol ; June 12 — Amphipols: polymers that keep membrane pro- This work was supported by Public Health Service grant teins soluble in aqueous solutions.
Rev Biochem ;79 July — Particular thanks are due to F. Science ;95 January — Infect Immun Appendix A. Supplementary data ;31 March 3 — Protection against an intranasal challenge by vaccines formulated with native and recombinant Supplementary data associated with this article can be found, in preparations of the Chlamydia trachomatis major outer membrane protein.
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